Chaperonins Facilitate KNOTTED1 Cell-to-Cell Trafficking and Stem Cell Function

Xu, X. M., Wang, J., Xuan, Z. Y., Goldshmidt, A., Borrill, P. G. M., Hariharan, N., Kim, J. Y., Jackson, D. P. (August 2011) Chaperonins Facilitate KNOTTED1 Cell-to-Cell Trafficking and Stem Cell Function. Science, 333 (6046). pp. 1141-1144. ISSN 1095-9203

URL: http://www.ncbi.nlm.nih.gov/pubmed/21868675
DOI: 10.1126/science.1205727

Abstract

Cell-to-cell communication in plants includes the selective trafficking of transcription factors and other signals through plasmodesmata. The KNOTTED1 (KN1) homeobox (KNOX) family transcription factors, which use this pathway, are essential for stem cell establishment and/or maintenance. Here we show that KN1 trafficking requires the chaperonin complex, which belongs to a group of cytosolic chaperones that fold specific substrate proteins. Genetic and physical interaction data show a functional relevance for chaperonins in KNOX family-dependent stem cell maintenance. Furthermore, tissue-specific complementation assays indicate a mechanistic basis for chaperonin function during the posttranslocational refolding process. Our study shows that chaperonins are essential for the cell-to-cell trafficking of a subset of mobile transcription factors and demonstrates the importance of chaperonin-dependent protein trafficking for plant stem cell function.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > chaperonins
organism description > plant
therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: 26 August 2011
Date Deposited: 13 Oct 2011 19:10
Last Modified: 07 Apr 2015 20:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15561

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