Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression

Prasanth, K. V. , Camiolo, M., Chan, G., Tripathi, V., Denis, L., Nakamura, T., Hubner, M., Spector, D. L. (2010) Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression. Molecular Biology of the Cell, 21 (23). pp. 4184-4196. ISSN 1059-1524

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URL: http://www.ncbi.nlm.nih.gov/pubmed/20881057
DOI: 10.1091/mbc.E10-02-0105

Abstract

Noncoding RNAs play important roles in various aspects of gene regulation. We have identified 7SK RNA to be enriched in nuclear speckles or interchromatin granule clusters (IGCs), a subnuclear domain involved in pre-mRNA processing. 7SK RNA, in association with HEXIM 1 and 2, is involved in the inhibition of transcriptional elongation by RNA polymerase II. Inhibition occurs via sequestration of the active P-TEFb kinase complex (CDK 9 and Cyclin T1/T2a/b or K) that is involved in phosphorylating the C-terminal domain of RNA polymerase II. Our results demonstrate that knock-down of 7SK RNA, by specific antisense oligonucleotides, results in the mis-localization of nuclear speckle constituents in a transcription-dependent manner, and the transcriptional up-regulation of a RNA polymerase II transcribed reporter gene locus. Furthermore, 7SK RNA transiently associates with a stably integrated reporter gene locus upon transcriptional down-regulation and its presence correlates with the efficient displacement of P-TEFb constituents from the locus. Our results suggest that 7SK RNA plays a role in modulating the available level of P-TEFb upon transcriptional down-regulation by sequestering its constituents in nuclear speckles.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > antisense
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > ncRNA
CSHL Authors:
Communities: CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL labs > Spector lab
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
Depositing User: CSHL Librarian
Date Deposited: 05 Oct 2011 13:32
Last Modified: 13 Oct 2015 16:12
PMCID: PMC2993747
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15508

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