The control of axillary meristem fate in the maize ramosa pathway

Gallavotti, A., Long, J. A., Stanfield, S., Yang, X. A., Jackson, D. P., Vollbrecht, E., Schmidt, R. J. (September 2010) The control of axillary meristem fate in the maize ramosa pathway. Development, 137 (17). pp. 2849-2856. ISSN 0950-1991

URL: https://www.ncbi.nlm.nih.gov/pubmed/20699296
DOI: 10.1242/dev.051748

Abstract

Plant axillary meristems are composed of highly organized, self-renewing stem cells that produce indeterminate branches or terminate in differentiated structures, such as the flowers. These opposite fates, dictated by both genetic and environmental factors, determine interspecific differences in the architecture of plants. The Cys(2)-His(2)zinc-finger transcription factor RAMOSA1 (RA1) regulates the fate of most axillary meristems during the early development of maize inflorescences, the tassel and the ear, and has been implicated in the evolution of grass architecture. Mutations in RA1 or any other known members of the ramosa pathway, RAMOSA2 and RAMOSA3, generate highly branched inflorescences. Here, we report a genetic screen for the enhancement of maize inflorescence branching and the discovery of a new regulator of meristem fate: the RAMOSA1 ENHANCER LOCUS2 (REL2) gene. rel2 mutants dramatically increase the formation of long branches in ears of both ra1 and ra2 mutants. REL2 encodes a transcriptional co-repressor similar to the TOPLESS protein of Arabidopsis, which is known to maintain apical-basal polarity during embryogenesis. REL2 is capable of rescuing the embryonic defects of the Arabidopsis topless-1 mutant, suggesting that REL2 also functions as a transcriptional co-repressor throughout development. We show by genetic and molecular analyses that REL2 physically interacts with RA1, indicating that the REL2/RA1 transcriptional repressor complex antagonizes the formation of indeterminate branches during maize inflorescence development. Our results reveal a novel mechanism for the control of meristem fate and the architecture of plants.

Item Type: Paper
Uncontrolled Keywords: Maize ramosa pathway Transcriptional repression Axillary meristems STEM-CELL MAINTENANCE TRANSCRIPTIONAL REPRESSION INFLORESCENCE ARCHITECTURE FLORAL MERISTEM ARABIDOPSIS GENE TOPLESS ENCODES PROTEIN DOMAIN
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > zinc finger proteins
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: 1 September 2010
Date Deposited: 30 Sep 2011 14:55
Last Modified: 11 Jan 2017 20:21
PMCID: PMC2938917
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15416

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