Illuminating plant biology: using fluorescent proteins for high-throughput analysis of protein localization and function in plants

DeBlasio, S. L., Sylvester, A. W., Jackson, D. P. (March 2010) Illuminating plant biology: using fluorescent proteins for high-throughput analysis of protein localization and function in plants. Briefings in Functional Genomics, 9 (2). pp. 129-138. ISSN 2041-2649

URL: http://www.ncbi.nlm.nih.gov/pubmed/20093306
DOI: 10.1093/bfgp/elp060

Abstract

First discovered in jellyfish, fluorescent proteins (FPs) have been successfully optimized for use as effective biomarkers within living plant cells. When exposed to light, FPs fused to a protein or regulatory element will fluoresce, and non-invasively mark expression and protein localization, which allows for the in vivo monitoring of diverse cellular processes. In this review, we discuss how FP technology has evolved from small-scale analysis of individual genes to more high-throughput techniques for global expression and functional profiling in plants.

Item Type: Paper
Uncontrolled Keywords: fluorescent proteins maize Arabidopsis high-throughput techniques global expression functional profiling TARGETED GENE-EXPRESSION ENHANCER TRAP LINES CELL-TO-CELL ARABIDOPSIS-THALIANA FUSION PROTEIN DEVELOPMENTAL PLASTICITY TRANSGENE EXPRESSION EPIDERMAL-CELLS APICAL MERISTEM ROOT-MERISTEM
Subjects: organism description > plant > Arabidopsis
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: March 2010
Date Deposited: 29 Sep 2011 20:24
Last Modified: 08 Apr 2015 13:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15397

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