Peripheral Blood Mononuclear Cell Gene Array Profiles in Patients With Overactive Bladder

Cheung, W., Bluth, M. J., Johns, C., Khan, S., Lin, Y. Y., Bluth, M. H. (2010) Peripheral Blood Mononuclear Cell Gene Array Profiles in Patients With Overactive Bladder. Urology, 75 (4). pp. 896-901.

URL: https://www.ncbi.nlm.nih.gov/pubmed/19775734
DOI: 10.1016/j.urology.2009.06.021

Abstract

Objectives: To investigate the utility of using peripheral blood mononuclear cells (PBMC) as a marker for patients with overactive bladder (OAB). Patients with OAB may suffer from varying degrees of symptoms such as frequency, urgency, nocturia, and incontinence; however, there is no definitive test for OAB at this time. Questionnaires may provide useful tools for screening patients for OAB but often clinicians may need to rely on more invasive procedures to confirm the diagnosis. We have previously demonstrated that PBMC can provide a reporter function in solid organ retroperitoneal disease. Methods: Twenty-one patients were assessed for OAB. PBMC was obtained from whole blood of the patients, and RNA was subjected to microarray gene chip analysis. Results: Microarray analysis revealed that 16 genes were differentially regulated (8 upregulated and 8 downregulated) in all patients with OAB in comparison with healthy controls. A sex-based analysis demonstrated 74 genes that were differentially regulated in males (25 upregulated and 49 downregulated), and 30 in females (13 upregulated and 17 downregulated). Of these platelet-derived growth factors, microfibrillar-associated protein and tropomyosin were downregulated in all sets that were analyzed. Conclusions: Microarray analysis revealed many genes that were differentially regulated in PBMC from OAB patients, including regulatory elements and genes encoding structural proteins, which may be important in regulating structural integrity of the bladder and supporting tissues. These data suggest that PBMC can provide a reporter function for patients with OAB and may serve as a diagnostic marker and elucidate genes involved in this condition. © 2010 Elsevier Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: Overactive bladder mononuclear cell gene array profiling
Subjects: diseases & disorders
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
organism description > animal > mammal > primates > hominids > human
CSHL Authors:
Communities: CSHL Cancer Center Shared Resources > Microarray Service
CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date Deposited: 29 Sep 2011 14:28
Last Modified: 28 Feb 2018 21:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15384

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