Arabidopsis TFL2/LHP1 specifically associates with genes marked by trimethylation of histone H3 lysine 27

Turck, F., Roudier, F., Farrona, S., Martin-Magniette, M. L., Guillaume, E., Buisine, N., Gagnot, S., Martienssen, R. A., Coupland, G., Colot, V. (2007) Arabidopsis TFL2/LHP1 specifically associates with genes marked by trimethylation of histone H3 lysine 27. Plos Genetics, 3 (6). pp. 855-866. ISSN 1553-7390

URL: http://www.ncbi.nlm.nih.gov/pubmed/17542647
DOI: 10.1371/journal.pgen.0030086

Abstract

TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 (TFL2/LHP1) is the only Arabidopsis protein with overall sequence similarity to the HETEROCHROMATIN PROTEIN 1 (HP1) family of metazoans and S. pombe. TFL2/LHP1 represses transcription of numerous genes, including the flowering- time genes FLOWERING LOCUS T (FT) and FLOWERING LOCUS C (FLC), as well as the floral organ identity genes AGAMOUS (AG) and APETALA 3 (AP3). These genes are also regulated by proteins of the Polycomb repressive complex 2 (PRC2), and it has been proposed that TFL2/LHP1 represents a potential stabilizing factor of PRC2 activity. Here we show by chromatin immunoprecipitation and hybridization to an Arabidopsis Chromosome 4 tiling array (ChIP-chip) that TFL2/LHP1 associates with hundreds of small domains, almost all of which correspond to genes located within euchromatin. We investigated the chromatin marks to which TFL2/LHP1 binds and show that, in vitro, TFL2/LHP1 binds to histone H3 di- or tri-methylated at lysine 9 (H3K9me2 or H3K9me3), the marks recognized by HP1, and to histone H3 trimethylated at lysine 27 (H3K27me3), the mark deposited by PRC2. However, in vivo TFL2/LHP1 association with chromatin occurs almost exclusively and coextensively with domains marked by H3K27me3, but not H3K9me2 or -3. Moreover, the distribution of H3K27me3 is unaffected in lhp1 mutant plants, indicating that unlike PRC2 components, TFL2/LHP1 is not involved in the deposition of this mark. Rather, our data suggest that TFL2/LHP1 recognizes specifically H3K27me3 in vivo as part of a mechanism that represses the expression of many genes targeted by PRC2.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: POLYCOMB GROUP-COMPLEXES HETEROCHROMATIN PROTEIN-1 DNA METHYLATION H3 METHYLATION EPIGENETIC CONTROL PLANT DEVELOPMENT BINDING-PROTEIN CPG METHYLATION FLOWERING TIME HP1 BINDING
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
organism description > plant
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date Deposited: 02 Sep 2011 21:29
Last Modified: 05 Jun 2013 14:15
PMCID: PMC1885283
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15264

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